The aim of this thesis is to analyze the facts known to the students and their interests in relation to 22 topics of sustainable development taught in the course Sustainable Development in Teaching Natural Sciences, which is part of the integrated undergraduate and graduate programme Biology and Chemistry Education, at the Department of Biology, Faculty of Science, University of Zagreb. In addition, the possible lack of knowledge and interest of students in certain topics of sustainable development have been investigated. For the purpose of this thesis, the responses written by the students in the so-called KWL tables (K - what I know, W - what I want to know, L - what I have learned) were analyzed during their participation in certain seminars within the course. The responses were specifically coded for the purposes of qualitative and quantitative analysis. Based on the responses in the completed KWL tables, students show a good understanding of environmental issues of sustainable development, but emphasize that they would like to learn more statistical data about environmental issues/challenges of sustainable development in order to get a more realistic picture of certain problems. Regarding the social issues of sustainable development, students show a good understanding of the topic, but they lack knowledge about ways to solve problems related to the social component of sustainable development, and they also indicate that they would like to know more about statistical data on social issues/challenges of sustainable development. The students' responses show that they are the least familiar with the El Niño / La Niña phenomena and the green and blue economies that they learn about in the course.