In cities, urban public transport is of particular importance. Its accessibility and connectivity can greatly affect the residents of a certain area. Weaker accessibility can be manifested in a greater distance of public transport stations or in a lower frequency of lines, which can cause significant organizational and financial problems and challenges for residents. The city of Split, as the second largest city in Croatia and the largest city in the Dalmatia region, has a strong gravitational influence at the regional level and as such contains the most functions and services for the residents. Considering the size of the city, the number of inhabitants and the role that the city plays, urban public transport is of great importance. Its good connectivity, accessibility and organization can greatly facilitate the daily needs of residents; therefore, this thesis analysed its accessibility in the city of Split. The analysed urban public transport includes a GIS analysis of public bus and rail urban transport. Accessibility includes the spatial arrangement and distance of bus and train stations from residential areas in the city, as well as the frequency of lines. The results of the analysis were obtained on the spatial differentiation of the city regarding the accessibility of urban public transport. The results indicate the favourable availability of city bus transport regarding residential areas in most city districts and the unfavourable accessibility of city rail transport, which is most evident in the lack of adequate infrastructure. At the end of the thesis, suggestions for possible improvement were given.