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Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices
IEEE Transactions on Biomedical Engineering, 71 (2024), 6; 1980-1992. https://doi.org/10.1109/TBME.2024.3358240
Rienmüller, Theresa; Shrestha, Niroj; Polz, Mathias; Stoppacher, Sara; Ziesel, Daniel; Migliaccio, Ludovico; Pelzmann, Brigitte; Lang, Petra; Zorn-Pauly, Klaus; Langthaler, Sonja; Opančar, Aleksandar; Baumgartner, Christian; Üçal, Muammer; Schindl, Rainer; Đerek, Vedran; Scheruebel, Susanne Više autora...

Institucijski repozitorij: Repozitorij Prirodoslovno-matematičkog fakulteta

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Rienmüller, T., Shrestha, N., Polz, M., Stoppacher, S., Ziesel, D., Migliaccio, L. ... Scheruebel, S. (2024). Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices. IEEE Transactions on Biomedical Engineering, 71. (6), 1980-1992. doi: 10.1109/TBME.2024.3358240

Rienmüller, Theresa, et al. "Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices." IEEE Transactions on Biomedical Engineering, vol. 71, br. 6, 2024, str. 1980-1992. https://doi.org/10.1109/TBME.2024.3358240

Rienmüller, Theresa, Niroj Shrestha, Mathias Polz, Sara Stoppacher, Daniel Ziesel, Ludovico Migliaccio, Brigitte Pelzmann, et al. "Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices." IEEE Transactions on Biomedical Engineering 71, br. 6 (2024): 1980-1992. https://doi.org/10.1109/TBME.2024.3358240

Rienmüller, T., et al. (2024) 'Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices', IEEE Transactions on Biomedical Engineering, 71(6), str. 1980-1992. doi: 10.1109/TBME.2024.3358240

Rienmüller T, Shrestha N, Polz M, Stoppacher S, Ziesel D, Migliaccio L, i sur.. Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices. IEEE Transactions on Biomedical Engineering [Internet]. 2024. [pristupljeno 02.10.2024.];71(6):1980-1992. doi: 10.1109/TBME.2024.3358240

T. Rienmüller, et al., "Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices", IEEE Transactions on Biomedical Engineering, vol. 71, br. 6, str. 1980-1992, 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:445483. [Citirano: 02.10.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:445483
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Naslov (engleski)Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes using Organic Photovoltaic Devices
AutorTheresa Rienmüller
AutorNiroj Shrestha
AutorMathias Polz
AutorSara Stoppacher
AutorDaniel Ziesel
AutorLudovico Migliaccio
AutorBrigitte Pelzmann
AutorPetra Lang
AutorKlaus Zorn-Pauly
AutorSonja Langthaler
AutorAleksandar Opančar
AutorChristian Baumgartner
AutorMuammer Üçal
AutorRainer Schindl
AutorVedran Đerek
AutorSusanne Scheruebel
Autorova ustanovaSveučilište u Zagrebu
Prirodoslovno-matematički fakultet
(Fizički odsjek)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Fizika
Sažetak (engleski)
Objective: This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltage-gated Cav1.2 channels ( I Ca,L) in Guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated I Ca,L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated I Ca,L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca 2+ channel blocker, nifedipine. Light-triggered I Ca,L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of I Ca,L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of I Ca,L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimal-invasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaIEEE Transactions on Biomedical Engineering
Brojčani podacivol. 71, br. 6, str. 1980-1992
p-ISSN0018-9294
e-ISSN1558-2531
DOIhttps://doi.org/10.1109/TBME.2024.3358240
URN:NBNurn:nbn:hr:217:445483
Datum objave publikacije2024
ProjektŠifra: UIP-2019-04-1753
Naziv (hrvatski): Mikro i nano-strukture za 3D opto-bioelektroniku
Naziv (engleski): Micro- and nano-structures for 3D opto-bioelectronics
Kratica: 3Doptobio
Voditelj: Vedran Đerek
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Installation Research Projects
URL dokumentahttps://ieeexplore.ieee.org/document/10473191/
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Javna napomena© 2024 The Authors. This work is licensed under a Creative Commons Attribution 4.0 License. For more information, see https://creativecommons.org/licenses/by/4.0/. Date of Publication: 18 March 2024.
Datum i vrijeme pohrane2024-07-24 10:18:54