prikaz prve stranice dokumenta Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15
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Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15
International Journal of Molecular Sciences, 25 (2024), 8; 4395. https://doi.org/10.3390/ijms25084395
Glunčić, Matko; Vlahović, Ines; Rosandić, Marija; Paar, Vladimir

Institucijski repozitorij: Repozitorij Prirodoslovno-matematičkog fakulteta

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Glunčić, M., Vlahović, I., Rosandić, M. i Paar, V. (2024). Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15. International Journal of Molecular Sciences, 25. (8). doi: 10.3390/ijms25084395

Glunčić, Matko, et al. "Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15." International Journal of Molecular Sciences, vol. 25, br. 8, 2024. https://doi.org/10.3390/ijms25084395

Glunčić, Matko, Ines Vlahović, Marija Rosandić i Vladimir Paar. "Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15." International Journal of Molecular Sciences 25, br. 8 (2024). https://doi.org/10.3390/ijms25084395

Glunčić, M., et al. (2024) 'Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15', International Journal of Molecular Sciences, 25(8). doi: 10.3390/ijms25084395

Glunčić M, Vlahović I, Rosandić M, Paar V. Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15. International Journal of Molecular Sciences [Internet]. 16.04.2024. [pristupljeno 06.10.2024.];25(8). doi: 10.3390/ijms25084395

M. Glunčić, I. Vlahović, M. Rosandić i V. Paar, "Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15", International Journal of Molecular Sciences, vol. 25, br. 8, Travanj 2024. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:391449. [Citirano: 06.10.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:391449
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Naslov (engleski)Novel Concept of Alpha Satellite Cascading Higher-Order Repeats (HORs) and Precise Identification of 15mer and 20mer Cascading HORs in Complete T2T-CHM13 Assembly of Human Chromosome 15
AutorMatko Glunčić
AutorInes Vlahović
AutorMarija Rosandić
AutorVladimir Paar
Autorova ustanovaSveučilište u Zagrebu
Prirodoslovno-matematički fakultet
(Fizički odsjek)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Fizika
Znanstveno / umjetničko
područje, polje i grana		INTERDISCIPLINARNA PODRUČJA ZNANOSTI
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
Sažetak (engleski)
Unraveling the intricate centromere structure of human chromosomes holds profound implications, illuminating fundamental genetic mechanisms and potentially advancing our comprehension of genetic disorders and therapeutic interventions. This study rigorously identified and structurally analyzed alpha satellite higher-order repeats (HORs) within the centromere of human chromosome 15 in the complete T2T-CHM13 assembly using the high-precision GRM2023 algorithm. The most extensive alpha satellite HOR array in chromosome 15 reveals a novel cascading HOR, housing 429 15mer HOR copies, containing 4-, 7- and 11-monomer subfragments. Within each row of cascading HORs, all alpha satellite monomers are of distinct types, as in regular Willard’s HORs. However, different HOR copies within the same cascading 15mer HOR contain more than one monomer of the same type. Each canonical 15mer HOR copy comprises 15 monomers belonging to only 9 different monomer types. Notably, 65% of the 429 15mer cascading HOR copies exhibit canonical structures, while 35% display variant configurations. Identified as the second most extensive alpha satellite HOR, another novel cascading HOR within human chromosome 15 encompasses 164 20mer HOR copies, each featuring two subfragments. Moreover, a distinct pattern emerges as interspersed 25mer/26mer structures differing from regular Willard’s HORs and giving rise to a 34-monomer subfragment. Only a minor 18mer HOR array of 12 HOR copies is of the regular Willard’s type. These revelations highlight the complexity within the chromosome 15 centromeric region, accentuating deviations from anticipated highly regular patterns and hinting at profound information encoding and functional potential within the human centromere.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 25, br. 8, 4395
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms25084395
URN:NBNurn:nbn:hr:217:391449
Datum objave publikacije2024-04-16
ProjektŠifra: IP-2019-04-2757
Naziv (hrvatski): Varijante u repetitivnim strukturama višeg reda u centromernim i NBPF dijelovima genoma čovjeka i viših primata
Naziv (engleski): Variants in higher order repeat structures in centromeric and NBPF regions of human and higher primates genomes
Kratica: HORVAR
Voditelj: Matko Glunčić
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
URL dokumentahttps://www.mdpi.com/1422-0067/25/8/4395
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Javna napomena© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Received: 6 March 2024. Revised: 8 April 2024. Accepted: 11 April 2024. Published: 16 April 2024.
Datum i vrijeme pohrane2024-07-24 10:24:19