prikaz prve stranice dokumenta Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
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Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
Current Biology, 32 (2022), 6; 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034
Velle, Katrina B.; Kennard, Andrew S.; Trupinić, Monika; Ivec, Arian; Swafford, Andrew J.M.; Nolton, Emily; Rice, Luke M.; Tolić, Iva M.; Fritz-Laylin, Lillian K.; Wadsworth, Patricia

Institucijski repozitorij: Repozitorij Prirodoslovno-matematičkog fakulteta

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Velle, K. B., Kennard, A. S., Trupinić, M., Ivec, A., Swafford, A. J., Nolton, E. ... Wadsworth, P. (2022). Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology, 32. (6), 1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

Velle, Katrina B., et al. "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features." Current Biology, vol. 32, br. 6, 2022, str. 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Velle, Katrina B., Andrew S. Kennard, Monika Trupinić, Arian Ivec, Andrew J.M. Swafford, Emily Nolton, Luke M. Rice, Iva M. Tolić, Lillian K. Fritz Laylin i Patricia Wadsworth. "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features." Current Biology 32, br. 6 (2022): 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Velle, K. B., et al. (2022) 'Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features', Current Biology, 32(6), str. 1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

Velle KB, Kennard AS, Trupinić M, Ivec A, Swafford AJ, Nolton E, i sur.. Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology [Internet]. 03.2022. [pristupljeno 18.02.2025.];32(6):1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

K. B. Velle, et al., "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features", Current Biology, vol. 32, br. 6, str. 1247-1261.e6, Ožujak 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:530398. [Citirano: 18.02.2025.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:530398
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Naslov (engleski)Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
AutorKatrina B. Velle
AutorAndrew S. Kennard
AutorMonika Trupinić
AutorArian Ivec
AutorAndrew J.M. Swafford
AutorEmily Nolton
AutorLuke M. Rice
AutorIva M. Tolić
AutorLillian K. Fritz-Laylin
AutorPatricia Wadsworth
Autorova ustanovaSveučilište u Zagrebu
Prirodoslovno-matematički fakultet
(Fizički odsjek)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Fizika
Sažetak (engleski)
Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the “brain-eating amoeba” Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria’s mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria’s spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCurrent Biology
Brojčani podacivol. 32, br. 6, str. 1247-1261.e6
p-ISSN0960-9822
DOIhttps://doi.org/10.1016/j.cub.2022.01.034
URN:NBNurn:nbn:hr:217:530398
Datum objave publikacije2022-03
ProjektŠifra: IP-2019-04-5967
Naziv (hrvatski): Ravnoteža sila i momenta sila u diobenom vretenu
Naziv (engleski): Balance of forces and torques in the mitotic spindle
Kratica: SpindleForceBalance
Voditelj: Nenad Pavin
Pravna nadležnost: Hrvatska
Financijer: Hrvatska zaklada za znanost
Linija financiranja: Research Projects
ProjektŠifra: KK.01.1.1.01.0004
Naziv (hrvatski): Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri
Naziv (engleski): Implementation of top research within the Scientific Center of Excellence for Quantum and Complex Systems, and Representations of Lie Algebras
Kratica: QuantiXLie
Voditelj: Hrvoje Buljan
Pravna nadležnost: eu
Financijer: Europska komisija
Linija financiranja: European Regional Development Fund
ProjektŠifra: KK.01.1.1.04.0057
Naziv (hrvatski): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini
Naziv (engleski): Inovative microscopy protocols for interdisciplinary research in biomedicine
Kratica: IPSted
Voditelj: Iva Marija Tolić
Pravna nadležnost: eu
Financijer: Europska komisija
Linija financiranja: European Regional Development Fund
ProjektŠifra: 855158
Naziv (hrvatski): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima
Naziv (engleski): Molecular origins of aneuploidies in healthy and diseased human tissues
Kratica: ANEUPLOIDY
Voditelj: Iva Marija Tolić
Pravna nadležnost: eu
Financijer: Europska komisija
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S0960982222000458
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Javna napomena© 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received: June 29, 2021. Received in revised form: November 2, 2021. Accepted: January 12, 2022. Published: February 8, 2022.
Datum i vrijeme pohrane2024-08-22 20:05:05