Cisplatin, platinum cordinated planar complex, is successful chemotherapeutic, used for the treatment of solid tumors. Binding of cisplatin to DNA results in the death of neoplasms, but application is limited by nephrotoxicity resulting in reduction or loss of kidney function. Of the numerous mechanisms that contribute to nephrotoxicity, oxidative stress is among the most pronounced. Iron is an essential element that the kidneys accumulate over time because it is not excreted. This increases the storage of iron in ferritin nanocages in the proximal tubules of the nephron and the risk of its release during cellular stress. How ferritin expression is altered by cisplatin treatment has been insufficiently investigated. The cells of the S3 section (pars recta) of the proximal tubules of the nephron in experimental animals experience the most damage. The aim of this work is to determine whether there is a change in the presence and distribution of both ferritin subunits in cisplatin-induced nephrotoxicity. After a single treatment with cisplatin (i.p. at a dose of 5 mg/kg), the nephrotoxic effect in male Wistar rats was expressed on the 5th day. Using specific antibodies, we monitored changes in the presence of light and heavy ferritin chains, which make up the nanocage, in archival samples of kidney tissue homogenates by western blot analysis. Using immunohistochemical analysis, we monitored their change in distribution on kidney tissue cryosections. We determined the change in the expression of nucleic acids using the end-point RT-PCR technique. An increase in the presence of light mRNA and light and heavy ferritin protein was found, as well as an increase and change in distribution, which indicates impaired ferritin metabolism as a possible cause of oxidative stress. The process probably starts from ferritinophagy, degradation in lysosomes, through the release of iron from ferritin to the iterative formation of hydroxide radicals, and further into ferroptosis. Control of the above, with a positive impact on the therapeutic function, is the desired outcome of this kind of results.