Mitosis or nuclear cell division is the process which segregates duplicated chromosomes to two daughter cells. During metaphase, sister kinetochores oscillate around the equatorial plate of the mitotic spindle until anaphase onset. Movements of sister kinetochores depend on the microtubule dynamics. A large number of proteins regulate microtubule dynamics, and one of them is kinesin-8. It is known that kinesin-8s move toward plus-end of microtubule and stimulate microtubule shrinkage. However, the mechanism by which kinesin-8 regulates kinetochore directional switch during oscillations is not well understood. This graduation thesis is focused on quantitative characterization of the dynamics of kinetochores and kinesin-8 (Kif18A) motor proteins in metaphase of the human U2OS cell line. Confocal microscope was used for live imaging of U2OS cell line with stable expression of CENP-A-GFP and mCherry-α-tubulin, and wild type U2OS cell line transiently expressing Kif18A-EGFP with addition of fluorogenic probe SiR-tubulin. I analyzed the position of kinetochores in the mitotic spindle and signal intensity of kinesin-8 during metaphase of mitosis. The results show that kinetochore oscillation rate is greater in interval further from anaphase onset and inner kinetochores oscillate less than inner kinetochores. Furthermore, I analyzed the occurrence of an angle between the line drawn through sister kinetochores and the line perpendicular to the equatorial plate named  angle. The  angle greater then 30° appears close to mitotic spindle center. Interkinetochore distance and  angle are negatively correlated. Lastly, transient expression of kinesin-8 reduces degree of sister kinetochores oscillation in metaphase of mitosis.