Tissue stem cells are undifferentiated cells that can be found in many tissues and organs such as bone marrow, peripheral blood, gut, liver, bone and cartilage, epidermis, brain, pancreas and eye. As all of the stem cells, tissue stem cells are also characterized by ability to renew themselves through division and potential to differentiate in several cell types found in tissue in which they are localized. According to that potential, they can be classified as multipotent stem cells. Considering their property of multipotency, tissue stem cells have important role in maintaining and renewing its host tissue. Because of the capability to generate new, completely differentiated cells, tissue stem cells have potential application in cell-based therapies as the treatment against hardly curable degenerative diseases. Until today, there are many discovered mechanisms of stem cell treatments that are successfully used in medical purposes. Those mechanisms are based on the transplantation of the cells generated by differentiation of the tissue stem cells in organisms whose tissues and organs have been destroyed because of the disease. However, there is still a lot of space for new cognitions in stem cell research and much more experiments and studies have to be done to enable full use of stem cells' potential.