Mussels are commonly probed bio-marker species in studies of anthropogenic influences on environment. Metal content in shells mostly depends on environmental conditions, since metals tend to accumulate through biomineralization, precipitation and adsorption processes. Concentration of incorporated metals in mineral structure of the shell changes slowly, in contrast to variable concentration of metals in mussels' tissue caused by changes in physical and reproductive conditions. Analytical spectrometric methods based on plasma source provide precise and simultaneous determination of metal concentrations in real samples. However, interferences caused by high-content calcium matrix can have a significant influence on quantitative determination of the metal content in samples, in a manner of increasing or decreasing total intensity of the observed spectral lines. In this study, concentrations of the eighteen elements were measured using ICP-AES method, in samples with hight-content calcium matrix, such as the Noah Ark's shell crust (lat. Arca noae, Linnaeus, 1758.). Comparison of the measured detection limits and linearity ranges for standard aqueous solutions and matrix-matched calibration solutions was used to test the presence of spectral and no-spectral inerferences. The chosen measuring conditions were applied on representative samples of Noah's Ark shells collected in Pašman channel and in Nature park Telašćica during several months in 2014. Satisfying analytical accuracy of applied analytical procedures and sample measurements was established in comparison with certified reference materials. The elemental profile of the shell samples was derived from measured set of data using chemometric tools, univariate and multivariate analysis. The impact of different environmental conditions on elements content in shell samples was observed by use of multivariate statistical method (PCA). Elemental profiling of mussel shell Noah's Ark provides an insight in species status as well as in environmental status on the observed locations.