Heavy metals pollution is one of the most important environmental problems, due of their toxicity, stability, and tendency to accumulate in living organisms. Therefore, heavy metals should be removed from the wastewater before it can be discharged into a natural recipient. Natural zeolitized tuff was proved to be a suitable material with high capacity binding of Cu 2+ ions from wastewater. The antibacterial activity of natural zeolitized tuff containing 2.60 percentage by weight (wt.%) Cu2+ were tested against bacteria Escherichia coli and Staphylococcus aureus after 1 h and 24 h of contact with zeolit in three different media, namely: Luria Bertani (LB) medium, synthetic wastewater and real wastewater. The copper – loaded zeolitized tuff showed antibacterial activity against Gram - positive bacteria Staphylococcus aureus and Gram - negative bacteria Escherichia coli, after 24 h of contact. The reduction of bacterial numbers was efficient in synthetic water (87,35 – 95,67%) and real effluent water (86,79 – 93,54%), while in the Luria Bertani (LB) medium the reduction was not efficient (5,54 – 10,69%). Since there was not leaching of toxic Cu2 + ions in the aqueous solution, Cu - loaded zeolite can be regarded as promising antibacterial material for disinfection of wastewater.