Helicobacter pylori is a Gram-negative spiral bacterium that colonizes the stomach cells, manages long-term bacterial infections (especially strain P12) and develops many diseases of the upper gastrointestinal tract. Overexpression of HP1026 (helicase responsible for recombinant activity) protein from P12 H. pylori strain, purification and crystallization using the "sitting drop" technique was performed. At the XRD1 beamline of the synchrotron "Elettra Sincrotrone Trieste", the quality of 20 crystals was investigated by X-ray diffraction on single crystals. The best diffraction data obtained had the resolution of 4 Å, in the hexagonal crystal system with unit cell parameters of a = 116.22(2), c = 238.38(2) Å. A very simple and successful method of cloning the crdA gene by creating the SUMO fusion protein was done, followed by protein overexpression, purification and crystallization of the CrdA protein from the H. pylori strain P12. The obtained result was a pure and concentrated solution of the CrdA protein which can be further used in crystallization experiments.Helicobacter pylori is a Gram-negative spiral bacterium that colonizes the stomach cells, manages long-term bacterial infections (especially strain P12) and develops many diseases of the upper gastrointestinal tract. Overexpression of HP1026 (helicase responsible for recombinant activity) protein from P12 H. pylori strain, purification and crystallization using the "sitting drop" technique was performed. At the XRD1 beamline of the synchrotron "Elettra Sincrotrone Trieste", the quality of 20 crystals was investigated by X-ray diffraction on single crystals. The best diffraction data obtained had the resolution of 4 Å, in the hexagonal crystal system with unit cell parameters of a = 116.22(2), c = 238.38(2) Å. A very simple and successful method of cloning the crdA gene by creating the SUMO fusion protein was done, followed by protein overexpression, purification and crystallization of the CrdA protein from the H. pylori strain P12. The obtained result was a pure and concentrated solution of the CrdA protein which can be further used in crystallization experiments.