This thesis deals with the in vivo pharmacokinetics-absorption, metabolism and distribution of polyphenolic compounds from the sloe (Prunus spinosa L.) flower water extract and the water extract of sloe in combination with a diet enriched by proteins, fat and carbohydrates in the organs (small intestine, liver, brain, kidney) of the mouse C57BL/6. Furthermore, the bioaccumulative potential of polyphenolic compounds in these organs was also investigated, as well as the bioavailable maximum concentration of polyphenolic molecules. In addition, major oxidative stress biomarkers (MDA, PC), antioxidant enzyme activation (SOD, GSH, CAT), and antioxidative capacity of selected tissues were investigated. The obtained results show that out of a total of 32 types of polyphenolic compounds found in the sloe flower extract, various types of polyphenolic compounds bioaccumulate by absorption and biodistribution within organs at different times which confirms the set hypothesis of selective preference for bioaccumulation and retention of a certain type of polyphenolic compounds within different organs. The results of the antioxidation system analysis have also shown which groups of polyphenols and their metabolites activate specific enzymes involved in the antioxidative defence system (SOD, GSH, CAT) and the measured antioxidant tissue capacity indicates that certain organs in the test groups exhibit a relatively high antioxidative capacity in relation to the animal control group. The results of this study contribute to a better understanding of the affinity of certain groups of polyphenols to certain tissues and organs with the fundamental aim of achieving a better antioxidative status of the organism.