prikaz prve stranice dokumenta Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability
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Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability
Molecular Biology of the Cell, 29 (2018), 11; 1332-1345. https://doi.org/10.1091/mbc.E17-11-0667
Klemm, Anna H.; Bosilj, Agneza; Glunčić, Matko; Pavin, Nenad; Tolić, Iva Marija

Institucijski repozitorij: Repozitorij Prirodoslovno-matematičkog fakulteta

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Klemm, A. H., Bosilj, A., Glunčić, M., Pavin, N. i Tolić, I. M. (2018). Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability. Molecular Biology of the Cell, 29. (11), 1332-1345. doi: 10.1091/mbc.E17-11-0667

Klemm, Anna H., et al. "Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability." Molecular Biology of the Cell, vol. 29, br. 11, 2018, str. 1332-1345. https://doi.org/10.1091/mbc.E17-11-0667

Klemm, Anna H., Agneza Bosilj, Matko Glunčić, Nenad Pavin i Iva Marija Tolić. "Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability." Molecular Biology of the Cell 29, br. 11 (2018): 1332-1345. https://doi.org/10.1091/mbc.E17-11-0667

Klemm, A. H., et al. (2018) 'Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability', Molecular Biology of the Cell, 29(11), str. 1332-1345. doi: 10.1091/mbc.E17-11-0667

Klemm AH, Bosilj A, Glunčić M, Pavin N, Tolić IM. Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability. Molecular Biology of the Cell [Internet]. 2018. [pristupljeno 24.12.2024.];29(11):1332-1345. doi: 10.1091/mbc.E17-11-0667

A. H. Klemm, A. Bosilj, M. Glunčić, N. Pavin i I. M. Tolić, "Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability", Molecular Biology of the Cell, vol. 29, br. 11, str. 1332-1345, 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:958693. [Citirano: 24.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:958693
Podaci o radu
Naslov (engleski)Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability
AutorAnna H. Klemm
AutorAgneza Bosilj
MBZ: 349215
AutorMatko Glunčić
MBZ: 256133
AutorNenad Pavin
MBZ: 204800
AutorIva Marija Tolić
MBZ: 260543
Autorova ustanovaSveučilište u Zagrebu
Prirodoslovno-matematički fakultet
(Fizički odsjek)
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Fizika
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Biologija
Znanstveno / umjetničko
područje, polje i grana		PRIRODNE ZNANOSTI
Interdisciplinarne prirodne znanosti
Sažetak (engleski)
During metaphase, sister chromatids are connected to microtubules extending from the opposite spindle poles via kinetochores, protein complexes on the chromosome. Kinetochores congress to the equatorial plane of the spindle and oscillate around it, with kinesin-8 motors restricting these movements. Yet, the physical mechanism underlying kinetochore movements is unclear. We show that kinetochore movements in the fission yeast Schizosaccharomyces pombe are regulated by kinesin-8-promoted microtubule catastrophe, force-induced rescue and microtubule dynamic instability. A candidate screen showed that among the selected motors only kinesin-8 motors Klp5/Klp6 are required for kinetochore centering. Kinesin-8 accumulates at the end of microtubules, where it promotes catastrophe. Laser ablation of the spindle resulted in kinetochore movement towards the intact spindle pole in wild-type and klp5Δ cells, suggesting that kinetochore movement is driven by pulling forces. Our theoretical model with Langevin description of microtubule dynamic instability shows that kinesin-8 motors are required for kinetochore centering, whereas sensitivity of rescue to force is necessary for the generation of oscillations. We found that irregular kinetochore movements occur for a broader range of parameters than regular oscillations. Thus, our work provides an explanation for how regulation of microtubule dynamic instability contributes to kinetochore congression and the accompanying movements around the spindle center.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaMolecular Biology of the Cell
Brojčani podacivol. 29, br. 11, str. 1332-1345
p-ISSN1939-4586
DOIhttps://doi.org/10.1091/mbc.E17-11-0667
URN:NBNurn:nbn:hr:217:958693
Datum objave publikacije2018
URL dokumentahttps://www.molbiolcell.org/doi/10.1091/mbc.E17-11-0667
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 3.0 (CC BY 3.0)
Javna napomena© 2018 Klemm, Bosilj, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted: 28 November 2017. Revised: 27 March 2018. Accepted: 29 March 2018. Published Online:31 May 2018.
Datum i vrijeme pohrane2020-08-27 20:22:15