The marine bacterium Vibrio gigantis was isolated from Kaštela Bay from wild early-phase biofilm and cultivated. The results showed that Vibrio gigantis can be used as an appropriate and versatile model species for adhesion dynamics and biofilm evolution research. NGS analysis confirmed the highest relative abundance of this species inside biofilm samples. Its predicted genetic potential confirmed the activity in surface adhesion, cell motility and quorum sensing – a bacterial communication for collective behaviour. The adhesion dynamics was successfully quantified in a newly designed experimental setup. Real-time adhesion was also quantified in two different geometries, a barrel-like container and a microchannel, showing a spontaneous nutrient, and oxygen-dependent adhesion, with a sudden and irreversible detachment. Proceeding with the colony evolution experiments under a constant resource supply, the colonies first grew exponentially and eventually dispersed from the surface. Dispersion was simultaneous and collective, leaving filamentous-form bacteria on the bottom. It was shown to be triggered at a certain substrate coverage, indicating the importance of the adhered bacteria concentration for a collective response to unfavourable conditions. The progression of this collective dispersion phenomenon was studied by measuring its dependence on the position along a microfluidic PDMS channel under controlled longitudinal laminar flux. In particular, simultaneous monitoring of two different positions in the channel allowed a direct observation of dispersion delay. Furthermore, 2D monitoring revealed both transversal and longitudinal time dependence of the dispersion, both presented as a front-like space-time pattern. This indicated that dispersion is indeed driven by interaction between bacteria. The fact that the dispersion dynamics is independent of colony growth pattern (clustering) rules out the contact and/or hydrodynamical nature of bacterial interaction. Thereby, it is concluded that the coordination of bacterial dispersion is of a biological nature, i.e. quorum sensing-like.