Honey is a product of high nutritional value, and many types of honey can be found on the market, from common types that appear every year, to rare unifloral types whose appearance depends on climatic conditions and the availability of nectar and/or honeydew or they appear only in a specific geographical area. In this study, the physicochemical parameters, the total phenolic content, and the antioxidant capacity of a rare unifloral type of ivy honey (Hedera helix L.) were determined with the aim of characterization and statistical analysis of the results tested the difference in the selected parameters according to the region of sample collection. In addition, the sensory characteristics were determined to obtain a more complete description of this honey type. Based on the obtained results, ivy honey is characterized by a low ratio of fructose and glucose (F/G), a high ratio of glucose and water (G/W), which indicates rapid crystallization of this type of honey, and high free acidity. The average total phenolic content was 146.70 ± 17.09 mg gallic acid/kg honey, and FRAP value was 295.51 ± 35.88 μM Fe (II). Statistical analysis showed that the samples from the two regions of the Republic of Croatia differ significantly in terms of water content, electrical conductivity, diastase activity and FRAP values. For description of this specific type of honey and confirmation of preliminary results, it is necessary to perform an analysis on a larger number of samples to get a better and more complete insight into the characteristics of ivy honey and to make a proposal for recommended values for quality parameters to confirm uniflorality.