With the advent of virtual and augmented reality, there is a need for a new kind of user interface and it is necessary to recognize if there is an effective use of existing pointing devices in such interfaces. The interaction in these environments is done in three-dimensional virtual spaces, but there is no standard that defines guidelines on how to evaluate physical pointing devices in such areas. However, there is a standard that defines guidelines for evaluating physical pointing devices in traditional two-dimensional environments. This thesis proposes an adaptation of the aforementioned standard with a goal to evaluate pointing devices in three-dimensional virtual spaces. As part of this thesis an application was implemented with the proposed adaptation in mind which can evaluate computer mouses, video game controllers and Leap Motion controllers in a simulated 3D environment. An empirical experiment was conducted in which the aforementioned three devices were evaluated with the addition of a touchpad and a smartphone (acting as a touchpad). The devices were evaluated in two separate environments, the first three mentioned above were evaluated in simulated 3D, while the other two devices were examined in 2D. The obtained results revealed that the mean throughput in the simulated 3D experiment was 0,75, while the 2D experiment result had a mean of 1,81. There was also a significant difference found between the three devices used in the 3D experiment, while the devices used in 2D had no significant difference in interaction efficiency.