Aim: Literature data suggest that infertile patients present worse periodontal status, which may be a factor that complicates conception. Matrix metalloproteinase 8 (MMP-8) is an enzyme in saliva that is directly related to periodontal inflammation. The aim of our study was to validate a chairside mouth rinse test for detection of active form of MMP8 (aMMP8) in a population of women with unexplained infertility compared to age-matched fertile women, with and without periodontitis. Our hypothesis was that infertile women will have worse periodontal health and more positive MMP8 tests, compared to fertile women. Materials and methods: The study included 50 healthy norm-ovulatory females ages 25-45 with strictly defined unexplained infertility, and 50 healthy norm-ovulatory women at the same age who have conceived and delivered naturally. All participants underwent clinical periodontal examination and a chairside aMMP-8 oral rinse test. One half of the participants in each group was diagnosed with periodontitis, while the other half was without periodontitis. Results: The sensitivity and specificity of the test for the detection of periodontitis in the group of infertile patients was 88% and 68%, in the group of fertile patients 84% and 72% and 86% and 70% in the total patient population. Infertile patients with periodontitis had less advanced periodontitis than the control group, although the difference was not statistically significant. Conclusion: Our results have shown that infertile patients had better periodontal status and less advanced periodontitis than fertile women of the same age. Sensitivity and specificity of the test increase with the degree of progression of periodontitis. Patients with periodontitis can be relatively well identified, but based on our results, the benefit of this test in the treatment of idiopathic infertility is questionable.