When they are among peers, children use various ways to express their needs, potentials, satisfaction or dissatisfaction, and they often tend to use non-verbal signals which their peers, along with the adults, should be able to understand. Non-verbal communication is in most cases unintentional, especially when it comes to pre-school children who use it almost unconsciously. The topic of this paper is the analysis of non-verbal communication of pre-school children, and how they use it while playing. Playing is the basic activity of pre-school children, and also one of their basic needs. In order to have a qualitative educational communication with children and an insight in their emotional states, needs and stands, it is important to observe and correctly interpret non-verbal signs and signals which happen during playing. By using various literature we will investigate the characteristics and functions of non-verbal communication, child's non-verbal signs and signals, the importance of playing and communication during playing and in that way interpret non-verbal signs. While playing, children transform and develop step by step. In that process their non-verbal skills are important because they can either block or encourage the development of independence, selfrealization and communicative competence of children, along with their creativity.