Working with children of early childhood education, it is very important to be aware of traditional cultural heritage. One of the most famous traditional string instrument is tambura. Over the past hundred years, it has become part of the wider cultural reality, and educators can use it in their work with children from kindergarten onwards. The aim of the paper is to use the example of the tambura to raise awareness of the importance of preserving Croatian traditional heritage from an early age and to describe how tambura can be used in kindergarten in working with children of early childhood education. Of course, apart from being an instrument, tambura can be shown to children through various activities: musical, artistic, research cognitive and in various games. The paper describes in detail. within the framework of examples of good practice, the activities with the tambura carried out in several kindergartens in the area of the city of Zagreb and the Zagreb County. Various incentives created for the exact age of children, as well as for encouraging their developmental tasks, are presented and described. Methodical forms of work are also described, in which childrenˈs creativity is encouraged through work methods, for example, dramatization, songs and games with singing. It furder captures childˈs attention, develops a sense of speech, hearing, rhythm and cultural values, encouraging at the same time the individuality of each child. The paper also describes childrenˈs interest in the subject of tambura, as well as their re-entry into activities related to tambura.