Introduction: Prostate cancer is the second most common cancer worldwide, with as many as 1,414,259 new cases per year and the first in terms of prevalence. It is followed by lung cancer (1). According to data from the Croatian Cancer Registry issued in 2021, prostate cancer has become the most common cancer among the male population in the Republic of Croatia and accounts for 18% of all newly diagnosed cancers, i.e. 2434 patients. The aim of this research was to examine how much men in the Republic of Croatia know about prostate cancer. Methods: A cross-sectional survey was conducted from January to April 2024. Respondents were men aged 40 to 70 years. Data on men's knowledge and attitudes about prostate cancer were collected through an online survey. Results: The research involved 105 men whose average age ranged from 40 to 50 years (51.4%), seventy-nine were married and the majority (60%) had a high school education. Most men did not know what the main function of the prostate was, had a low level of knowledge about risk factors for prostate cancer, and 79% of them stated that they did not receive information about prostate cancer from their doctor, but from other sources. As a reason for postponing the digitorectal examination, 44.4% of men said they did not consider it necessary or were ashamed. 75% of them rate their own information about prostate cancer as insufficient, and the majority (91.7%) are interested in preventive examinations. Conclusion: The lack of information about prostate cancer and preventive examinations among men in the Republic of Croatia results in low level of knowledge about it. An effectively designed and implemented educational program can help identify the needs and priorities of the target population.