Introduction: Telemedicine (distance treatment) is defined as a way of performing medicine that involves the use of IT technologies, i.e. the transmission of medically relevant data at a distance. It enables better healthcare choices, increases the quality and efficiency of emergency services, shortens diagnosis time and saves costs for both doctors and patients by optimizing clinical procedures and reducing travel costs to hospitals. Objective: To investigate the perception of the usability of telemedicine in primary health care in the Republic of Croatia. Methods: A cross-sectional study was conducted. Data on the perception of the usability of telemedicine in primary health care in the Republic of Croatia were collected using an anonymous questionnaire in the clinics of the Zagreb-West Health Center and the Health Center of the Ministry of the Interior Affairs of the Republic of Croatia. Data were collected using a questionnaire from the article "Development of the Telehealth Usability Questionnaire (TUQ)" by Bambang Parmanto, Allen Nelson Lewis Jr., Kristin M. Graham, Marnie H. Bertolet (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4985278/). 200 patients received and filled the questionnaire in paper form when visiting the doctor. After completing the questionnaires, the collected data were computer processed. Results: 200 respondents were included in the research, of which 66 were male, while 134 were female. An equal share of respondents was included from the Zagreb-West Health Center and the Ministry of Internal Affairs Health Center of the Republic of Croatia. Most of the respondents used the telemedicine system and it was detected that younger ones use the benefits of telemedicine more often than older patients. Conclusion: It was found that younger respondents use the advantages of telemedicine more often than older people and all respondents used the telemedicine system in one period.