Introduction: Oxygen is used in hypoxemic patients and as such is an important part of daily clinical practice, diagnostic procedures, and inpatient and outpatient therapy. Less well known is the fact that during transport by ambulance as many as 34% of patients receive oxygen, while on average only 15-17% of hospitalized patients receive oxygen at some point in hospitalization. Unfortunately, there are still a number of misconceptions about the use of oxygen today. Among them is the opinion that not enough oxygen can be given, so the danger of excessive oxygen supply is not even understood. The importance of oxygen in terms of saving lives cannot be overemphasized, nor is it necessary to raise awareness of its side effects. Objective: To investigate the common practice in the application of oxygen related to the selected methods and type of oxygenation in individual emergencies of patients among emergency medical staff at different work sites. Materials and methods: A multicenter study was conducted on 129 respondents, through anonymous, online surveys over a 3-month period. All respondents volunteered to access the online survey questionnaire. Respondents were adults, both sexes, employed in different work places. The questionnaire consisted of 30 questions. The collected data was processed in the online query form "Google Forms". The collected data are presented in graphs, tabular and numerical. Different results, compared by statistical analyzes (Chi-square test) from frequency, were accepted as significant with significance less than 0.05 (P <0.05). Results: In the study have participaded more female respondents (71.3%; male 28.7%). The largest number of respondents is aged 26-39. Available oxygen cylinders were reported by 58.9% of respondents. In a transport vehicle, 83.7% of respondents can use oxygen. Oxygen therapy is used by 82.2% of subjects according to the measured values of peripheral saturation. In patients without chronic obstructive pulmonary disease (COPD), an equal number of subjects still administer oxygen with SpO2 saturation greater than 94%. (34.9%) while in patients with COPD most subjects use oxygen therapy according to the recommendation of BTA (SpO2 88-92%), is 94.1% (P <0.05). The largest number of respondents recorded the beginning and end of oxygen therapy on the list of patients (73.6%), while 26.4% still do not register them. Discussion: The approach of health professionals in the application of oxygen therapy is still not uniform. The difference is especially noticeable when it comes to achieving the so-called. targeted saturation of patients on oxygenation. Almost all health professionals have oxygen available. In their work, they regularly record the application of oxygen therapy, which is extremely important. Conclusion: The use of oxygen is of particular importance for patients, especially in outpatient settings. Healthcare professionals indicated that they have valuable knowledge about the application of oxygen therapy as well as that they follow modern guidelines in its application. No, regardless of this knowledge, there must still be a tendency to improve the existing standards in oxygen therapy as a constant implementation of education in the field of oxygen application.