Down syndrome is a genetic disorder in which an extra chromosome occurs during cell division. Children with Down syndrome have specific physical characteristics, various cognitive impairments, as well as numerous health problems. Some of the physical characteristics are a small mouth and nose with a disproportionately large tongue, a round face, hypotonia, short hands, a wide neck, abnormally shaped ears, and many others. During the preparation of the paper, research was conducted with the aim of determining the knowledge of the general population about the main characteristics of Down syndrome tests and the perception of children who have this disorder. A total of 256 people participated in the research, of which 197 (77%) were female, and 59 (23%) were male. The largest number of participants were aged 21-30, i.e. 154 of them (60.2%). Most of the participants have completed secondary education, that is 138 of them (53.9%). The results showed that most of the participants knew that Down syndrome is a genetic disorder, i.e. 243 of them (94.9%), while 248 of them (96.9%) said that it cannot be cured. 223 participants (87.1%) chose the correct characteristics of children with Down syndrome among the answers offered. About half of the participants declared that there are no children with Down syndrome in their environment, and regarding personal attitudes, most participants, 175 of them (68.4%) chose the offered answer that children with Down syndrome radiate love. Based on the obtained results, it can be concluded that the public is well educated about this disorder, and most of the participants correctly answered the questions about the characteristics of Down syndrome. The connection between more frequent meetings with children who have Down syndrome and the perception of personal attitude towards such children came to the fore. The care of a child with Down syndrome is aimed at improving the quality of life as well as raising public awareness of this disorder.