Considering the nature of contemporary business practice, organizations are becoming more and more aware of how collaboration within the organization has become significantly more important than before. Today’s business requires commitment of all organization members in order to perform a task as quickly as possible without compromising quality, because the key to success lies precisely in speed, flexibility and adaptability to changes in the market. In organizations that have successfully implemented collaboration in their business model, the collaboration is visible at all levels within the organization as well as in its organizational culture. What is crucial for a successful implementation of collaboration within the organization is the analysis of a number of factors that influence success such as acceptance of diversity among employees, the level of commitment of organization members to teamwork, trust, and mastery of required abilities. In writing this thesis, I have attempted to use the available literature to outline and comment on the facts relating to intra-organizational collaborative behavior and the way it affects performance of socio-economic organizations. The structure of this thesis is organized in several sections. The first section sets out the definitions of basic terms associated with collaboration and describes the importance of collaboration and the advantages and challenges it brings. The second half of the thesis presents an overview of all essential factors which may have different effects on the success of intra-organizational collaborative behavior. The final section outlines a couple of business case studies to demonstrate how the theories elaborated in this thesis actually work in practice.