Ecotourism belongs to tourism of special interests. He focuses on nature and its conservation. Ecotourism began to develop 130 years ago and was then only associated with parks that played a significant role in achieving the main goals. The first national park in the world is Yellowstone. As awareness of nature protection increased, nature protection associations began to be established. Eco-tourists are most aware of nature and its preservation. There are three types of ecotourists and it differs in the following categories: Eco specialists, general ecotourists and medium type ecotourists. As it exists for everyone, so there are principles for ecotourism that everyone must adhere to, so as not to disturb nature and its flow. This seminar presents global examples of good practice such as: Cambodia, Slovenia, Quadra Island and Haida Gwaii Islands. The paper studies ecotourism in Croatia and Istria. At the end of the whole paper there is a case study with research results. Istria is developing special development programs. Two programs implemented in Istria are: GeoIST3A and Eco Domus, and this program increase environmental and social responsibilities in tourism. With these programs, a natural heritage that has not been used so far is put into operation. Through the research that was conducted, the development potential of ecotourism in the Brijuni National Park was presented. Brijuni is developed through visitor education, sports and recreation content, but also through eco-friendly accommodation. It can be concluded that Brijuni National Park is developing ecotourism. Eco accommodation and Eco beaches with their realization will be raised to a new level with the help of EU funds. The hypothesis set in the paper is confirmed. The goal and hypothesis of the paper are fulfilled. Keywords: culture, tourism, eco-tourism, Brijuni, eco-tourist, Istria.