The subject of research in this paper relates to the Tourist walking trails of the Istrian County. The problem of this research is to analyze the current offer of walking tourism in the Istrian County and the possibilities of its further development. Increasingly represented forms of tourism of special interest are visible through sports and recreational facilities that represent new guidelines for tourism development. The connection between a healthy lifestyle and free time is visible by the valorization of sports and recreational activities in the function of tourism, which creates an authentic experience of the destination. Walking tourism is based on the basic human physical activity of walking, and due to its accessibility it is becoming an increasingly popular form of spending free time. Various forms ofwalking tourism represent a solution for the unbalanced spatial development of tourism, the negative impact of seasonality and the preservation of cultural and historical heritage and traditional values. The analysis of natural and anthropogenic tourist attractions and walking trails established that the area of Istria County has a developed walking infrastructure. Tourist activities in the County of Istria are seasonal and the analyzed factors represent the potential for further development of walking tourism in areas that achieve less economic benefit from more developed tourist areas. At the same time, favorable climatic conditions are imposed as a key factor in the development of year-round tourism. The increasingly intensive positioning of the Istrian County as a destination for walking tourism is also visible through marketing activities at the county level. In addition to thematic chapters, attention is paid to the results of research on walking microregions according to given criteria. The analysis of the given criteria identified the advantages and disadvantages of individual micro-regions and proposed guidelines for the further development of hiking tourism. The conducted analysis also determined the spatial dichotomy of tourist activities and the representation of tourist beds, which are manifested through the influence of seasonality and the load on the coastal area. The research concluded that the development of walking tourism is conditioned by elements of geographical space and that the County of Istria has natural and anthropogenic resources for further development of hiking infrastructure and improving the socio-economic structure of the domicile population through sustainable development.