Comic books are works with a rich cultural concept and a unique way of addressing a wide range of topics. Due to the specificity of the medium, they are appealing to people of all ages. Each public library contains a collection of comic books, some of which count only a few dozen titles, up to those consisting of thousands of units and are highlighted by their size and quality. However, there are various difficulties which occur during formal processing of comic books in libraries, subject analysis being one of the most problematic. Subject analysis consists of a series of steps used for describing the content of a publication, and its purpose is to enable subject access to information that is becoming increasingly needed in today's modern information age. One of those steps is subject indexing, the process of marking the content of a document with subject terms. Because of its complexity, librarians are often hesitant about it, especially when it comes to unconventional materials such as comic books. In this paper, two researches were conducted (first one among librarians and the other among users). Their aim was to analyze the present condition of subject analysis of comic books in Croatian public libraries and the user perception of subject indexing of comic books with the purpose of pointing out that the current approach to subject indexing of comic books needs to be adjusted in order to enable better subject access in library catalogs.