The species Malassezia pachydermatis is a zoophilic yeast that changes from a commensal to a pathogen when conditions are favorable for its excessive reproduction, and in dogs and cats it most often causes inflammation of the external auditory canal or inflammation of the skin. Topical application of antimycotics from the azole group, such as miconazole, is a successful method of treatment, but often recurring infection and the development of a chronic course of the disease occur, with an increasing percentage of resistant strains of this yeast species being isolated. The aim of this research was to determine the sensitivity of M. pachydermatis species to the antifungal agent miconazole using the broth microdilution method. For this purpose, 40 strains isolated from dogs and cats were tested by adapting the standardized microdilution procedure described by document E.DEF 7.3.2 of the European Committee for Antimicrobial Susceptibility Research. Considering the lipophilic characteristics of the genus Malassezia, Sabouraud broth with the addition of 1% polysorbate Tween 80 was used. The final density of the inoculum suspension was 0,5 to 2,5 x10⁵ colony forming units per milliliter. The microtiter plates were incubated for 72-96 hours at 37°C, and the reading was performed with a spectrophotometer. The range of minimal inhibitory concentration (MIC) values obtained was from 0.5 to >32 mg/L. Antimicrobial resistance was determined in two strains whose growth was not inhibited even by the highest antifungal test concentration of 32 mg/L by ≥50%. This research points to the need for the development of a standardized broth microdilution procedure for the genus Malassezia and for additional research aimed at clarifying the role of resistance in these species.