Psittacine beak and feather disease represents one of the most important diseases in wild birds, as well as in pet birds. It affects feathers, beaks and claws of the birds, although it is often a fatal disease, especially in young birds, while in adult birds it can cause chronic problems which significantly diminishes the quality of life in the infected birds. It is caused by circovirus that is immunosuppressive and hepatotoxic, which consequently leads to secondary infections and death. The disease is distributed all around the world thanks to its high contagiousness. The purpose of this study was to determine the prevalence of circovirus in parrots in captivity through analyzing samples obtained from the Bird Clinic at the Faculty of Veterinary Medicine, University of Zagreb, and from a parrot breeder. We have collected 131 sample originating from various psittacine species – 20 fecal samples, 24 feather samples, 2 cloacal swabs and 9 organ samples (=55) were collected form the Clinic, while 40 fecal samples and 36 feather samples (=76) were collected from the breeder. We have determined that 16 out of 36 (44,4%) examined birds from the Clinic tested positive for PBFD. Half of the positive birds did not show any clinical signs of PBFD. Interestingly, we have determined 5 out of 54 (9,26%) positive birds from the breeder, and all positive birds were paired with birds which tested negative for PBFD. Considering the results of this research, it would be beneficial to introduce regulatory measures for continuous monitoring of circovirus presence and transmission in Croatia, with emphasis on breeders and pet shops.