This study investigates the incidence of some pathological changes and causes of deaths in zoo animals in the Republic of Croatia, whose carcasses were submitted to pathological analysis to the Department of Veterinary Pathology at the Faculty of Veterinary Medicine in Zagreb in a six years period (1.1.2009 - 31.12.2014). Out of a total of 247 processed animals, 243 were from the Zoological Garden of Zagreb, and only 4 from the Zoological Garden of Osijek. The most common submitted animals were mammals (127), birds (60), reptiles (57) and les common amphibians (3). There were three different causes of death (septicemia, amyloidosis and enteritis) diagnosed in the amphibians. In the reptiles, the most common cause of death was infection, and traumas and degenerative diseases were also among more prevalent causes. In the birds, the most frequent cause of death was trauma, followed by infection and degenerative changes. In the mammals, the most common cause of death was lung inflammation, followed by septicemia, and trauma and inflammation of the intestine. The overall results of our research show that the causes of deaths of animals in the Zoo of Zagreb are not significantly different from the causes of deaths mentioned in the literature, and that infectious diseases prevail, while the proportion of traumas and parasitic invasions is lower compared to the results presented in the literature available to us. The results of our study confirms application of pathological for better dissases control and prevention of spreading of zoonotic diseases.