Artificial insemination is the procedure of depositing of sperm in the female reproductive organs, after which fertilization naturally takes place inside the fallopian tubes. Benefits of AI are numerous and therefore the interest of breeders for the AI worldwide is growing rapidly. The procedure is artificial insemination includes obtaining semen from a dog, semen evaluation, determination of the optimal time for insemination of bitches and election of the method of depositing of semen into the reproductive organs of bitches. Semen which is used for artificial insemination can be fresh, chilled or frozen. There are two basic methods of AI , vaginal deposition and transcervical intrauterine artificial insemination, while for the intrauterine there are two techniques, non-surgical, while using a Norwegian / Scandinavian catheter with or without an aid of endoscope, and surgical or laparoscopic techniques . The success of artificial insemination depends on choosing the correct time for insemination, use of quality seeds, proper handling and proper election of the AI method.