This research retrospectively processed archive data of the Laboratory for diagnostics of bacterial diseases of Department for microbiology and infectious diseases with clinic of Veterinary faculty in Zagreb, during time period of four years (2015- 2019). Results of bacteriological examination of diagnostic material originating from dogs with clinical signs of respiratory disease were analysed. Aim of the study was to determine how often bacterial agents were present, were they cultivated in pure or mixed culture, which bacterial species were most often detected and to define their antimicrobial susceptibility. Bacterial agents were found in 72,3% of all cases – 55,5% in pure and 45,5% in mixed culture. Most commonly isolated bacterial species were B.bronchiseptica, followed by S.pseudintermedius, E.coli and coagulase-negative Staphylococcus sp. Antimicrobial susceptibility was assesed for each one of the bacteria mentioned above, which indicated difficulty in choosing proper empirical therapy for the patients. Moreover, it proved the fact that objective diagnostics and bacterial culture is sometimes essential in selection of appropriate antimicrobial therapy.