PET scan has is highly important method in every day diagnostic and research in which aplication on laboratory animals has a great importance in development of the same. But, there is continuous need for new laboratory models which will suit better to PET scan application. Chicken embryos showed to be model for PET scan application in last few years, but without defined all possible methods and Chicken embryos have in recent years proved to be an interesting model for research in the field of PET, but all the methods and conditions of application of radiopharmaceuticals to them have not been fully defined. In this study, we investigated the possibility of applying fluorodeoxyglucose (18FDG), the most commonly used radiopharmaceutical, in chicken embryos of heavy breed on 13th day of incubation. Mentioned radiopharmaceutical was applied by different methods, iv into the blood vessels of chorioallantoic membrane (CAM), into the embryo or by drip into the air chamber. Application was followed by recording during the period of about one hour, in case of iv administration or into the embryo immediately, or in case of dripping after one hour of uptake. PET scan was followed by reconstruction, processing and analysis of the resulting images. The results showed that the iv application of FDG into the CAM blood vessel gave the best results, although not in all cases of applications, while other methods were less effective. IV applied FDG ensure the rapid dissemination and high-quality imaging of whole embryo and organs of interest (brain, heart and kidney). Confirmed is the need for chemical immobilization of embryos during recording because of the constant movement. Further research in the direction of PET application for imaging of changes in the chicken embryo is needed, especially after several days of incubation after the application of microorganisms or neoplastic cells, which precise monitoring will be enabled by application at an embryo early age of 13 days.