Musculoskeletal pain is a common problem in middle-aged women who go through a series of physiological and hormonal changes that significantly affect their quality of life. The musculoskeletal system plays a key role in the mobility and functionality of the body, but it is subject to various diseases and pains, which become more pronounced with age. Physical activity is defined as any body movement that involves muscle contraction and energy consumption greater than at rest. Although physical activity is known for its broad health benefits, its relationship with musculoskeletal pain varies depending on the type, intensity, and frequency of exercise, as well as individual factors such as hormonal status and preexisting conditions. Regular physical activity of low to moderate intensity can relieve musculoskeletal pain by increasing muscle strength, improving joint function, and reducing inflammation. In addition, such activity can improve general mobility and reduce the risk of further injury, thus contributing to a better quality of life. Physical activity as a therapeutic intervention for the management of musculoskeletal pain in middle-aged women requires careful consideration of individual needs and limitations. Tailored exercise programs that take into account hormonal status, type and intensity of physical activity are key to optimizing pain and improving quality of life in this population. In addition to physical activity, other aspects such as proper nutrition, maintaining a healthy body weight and psychological support are also important, which together contribute to the overall health of the musculoskeletal system. Physical activity plays a key role in health promotion, prevention and treatment of musculoskeletal pain. The aim of this work is to determine whether there is a connection between the level of physical activity and the appearance of pain in the musculoskeletal system in middle-aged women.