Introduction: There is a decline of physical activity levels among general population, including young adult people. Consequently, physical inactivity is affecting people's mental and physical health, causing different types of morbidities. The purpose of this study is to assess levels of physical activity of students enrolled at College of Applied Sciences „Lavoslav Ruzicka“ in Vukovar, students' attitudes regarding self-esteem and to investigate association between selected variables. Methods: Research was conducted in January, 2018, using anonymous structured web-based survey. It included 101 participants. Results: High levels of physical activity were recorded in 39,6% of participants. Highly active participants evaluate their health as better (p=0,002) and have higher levels of selfsatisfaction (p=0,008) comparing to participants with low or medium levels of physical activity. Students with higher body mass index do not perceive their health as worse in comparison to other students (p=0,082). Physiotherapy students do not have higher levels of physical activity than students enrolled in other degree courses which are non-health-related (0,482). Disscusion: It is essential to raise awareness of young people regarding the importance of physical activity with the goal to prevent morbidities of the locomotor, cardiovascular and other organ systems.