In order to ensure higher crop yields in the intensive agricultural production, a large amount of fertilizer is used, which is an increasing problem because some forms of nitrogen, such as nitrate ions, are prone to leaching into deeper soil and groundwater. Research was conducted at the Jazbina experimental vineyard as part of the SUPREHILL project. Disturbed soil samples were collected in the field, and the physical and chemical characteristics of the soil (texture, organic matter content, pH, E.C.) were determined at three different positions on the hillslope. Wick lysimeters were installed on the slope in 3 repetitions at 3 positions: at the top, middle and bottom of the hillslope, a total of 18 lysimeters. Water samples were collected from the leachate of the wick lysimeters every two weeks. Nitrates in water samples were determined spectrophotometrically using continuous flow analyzer. Differences in nitrate concentrations relative to slope position were analyzed using the ANOVA test in the SAS program. The obtained results indicate that there were no statistically significant differences, although it can be seen that the nitrate concentration in the leachate at the top of the hillslope is lower than the nitrate concentration in the middle and bottom of the hillslope.