Given that in certain museums, such as Natural history museums around the world, biodiversity and stuffed animals can be seen for centuries, some museums decided to go in a new and different direction and have opened their doors and spaces to living animals. Temporary or permanently residing in the museum, they offer visitors a different experience in viewing exhibitions or are helpful to the museum staff. In addition to displaying live animals in their temporary or permanent museum settings, some museums and related institutions have decided to use the abilities of animals in the preservation of museum exhibits. In contrast their special "employees" have become part of the museum staff, thus they serve as an additional form of preservation and conservation of museum objects. Everything mentioned, as well as supported by examples, is analyzed in more detail in this paper. This graduation thesis was written to increase awareness of animals that are part of our ecosystem and their welfare for people in various aspects. Special attention is given to living animals that live in the museum daily or are temporarily shown as part of an exhibition. As well as to temporary visits by pets, which until recently, access was utterly prohibited. The needs of visitors change over time, so the museum is the one that should listen to their needs and accordingly embrace new approaches; due to digitization and more modern technology, a visit to the museum is sometimes not even necessary, but also because of a change in the consciousness of people who strive to connect with nature.