The aim of this work was to investigate the influence of silk fibroin (SF) and different content of antibiotic Cefuroxim (CFU) on the viscoelastic, mechanical and thermal properties of PCL/FS/CFU electrospun carriers in order to prevent eye infections after surgery. Electrospun 18% PCL and 10% FS fiber scaffolds are prepared by electrospinning with the addition of the CFU of 4,8 and 12 wt%. The electrospinning process was followed by characterization of the electrospun scaffolds. The structure of electrospun scaffolds was determined by FTIR-ATR spectroscopy, mechanical properties by tensile testing, thermal properties by thermogravimetric analysis and viscoelastic properties by dynamic-mechanical analysis. With the addition of FS and CFU and the increase of the CFU content, the peak in the glass transition region is reduced relative to the electrospun PCL carrier, but the peak maximum increases with the increase in the CFU content. Based on the increase in Tg, it can be concluded that the FS crystal domains are located in the amorphous phase of the electrospun PCL carrier and an interaction occurs between the PCL and β-plate crystals from FS, thus reducing the mobility of the amorphous segments in the PCL carrier. The addition of FS in an 18% PCL electrospun carriers results in an increase in tensile strength and a decrease elongation at break. Increasing the content of CFUs in PCL/CFU/FS electrospun carriers results in a decrease in tensile strength and elongation at break. On the FTIR spectra of PCL/FS/CFU electrospun carriers, three characteristic bands attributed to the CFU can be seen. Thermal stability is reduced by the addition of 10% FS in 18% PCL electrospun carrier as well as electrospun PCL/FS/CFU carriers by the addition of FS and CFU.