The tendency in modern organizations is to achieve maximum quality of the products and services at minimum costs of production which means at the same time achieving greater profitability of the organization. In order to realize this goal, i.e. to achieve business excellence it is necessary to apply the quality management system and to implement it successfully in the organization system. Since the customers’ satisfaction becomes the measure for quality, a modern approach to quality refers not only to the quality of the finished product but rather also to all the functions of business operation. The paper describes the systems of Total Quality Management (TQM) and Six Sigma (6σ). It also presents the characteristics of the ISO 9000 standard which seems to represent a good base for the implementation of the mentioned systems. Since the described methodologies TQM and Six Sigma are rarely interpreted in the correct manner, a special part of the paper is dedicated to their comparison. The key contribution of the paper lies in the presentation of the research results of the implementation of both methodologies in graphical industry in the Republic of Croatia, as well as the application of the ISO 9000 standard. The analysis of the results shows that the implementation of TQM and Six Sigma in Croatia is still absent, and it may be seen that even the ownership of certificates according to ISO 9001 standard fails to be at a desired level.