Competitive and interactive competitive gaming (E-sport) is a growing activity and interest today. An increasing number of people play video games professionally or amateurs, invest in their development and the development of E-sports, or regularly follow and support various tournaments and leagues organized around the world. Previous research points to the importance of continuous examination and study of various parameters related to performance and the physiological, psychological and cognitive state of players in different stages of playing, especially in the professional context of E-sport. This paper focuses on a comprehensive review of the findings so far on the relationship between various factors and the physiological and psychological status of the participants. Specifically, this thesis includes determinants of stress reactions and changes in heart rate, blood pressure, electrodermal activity, cortisol level, glucose level and vocal expression, depending on various factors (e.g. the nature of the game, the outcome of the game, the expertise of the player, the pressure experienced, the nature of the game instruction, etc.) and the very act of participating in playing video games. Additionally, the connection between nutrition and E-sports was analyzed. Professional E-sport players experience significant amounts of internal and external pressures and stress reactions (tension, anxiety, physiological reactions). The most frequently mentioned stressors that players experience are performance pressure, the question of financial income, the influence of audience and the media, the difficulty of balancing work and private life, which often results in emotional exhaustion of players (i.e. burnout). Research indicates significant changes in the mentioned physiological parameters when participants participate in playing video games and increased activation of the sympathetic nervous system. Also presented are the findings of a comparison of different video games, depending on their nature and character, where video games with violent and martial content lead to greater physiological activity, compared to less aggressive or no aggressive video games. The paper proposes practical guidelines for improving the performance and well-being of E-sportsmen and implications for future research. In the field of E-sports, it is important to continue to research and further expand the existing knowledge.