Introduction: Complementary and alternative medicine are becoming more popular globally. Interest in dietary supplements became more pronounced during COVID-19 pandemic. Research goals: To research frequency of healthcare professionals’ consumption of dietary supplements, and COVID-19 effect on the supplement consumption. Participants and methods: The survey was conducted during December 2020 and January 2021. Data was collected voluntarily through anonymous surveys optimized for this research. The inclusion criterion in the survey was that the respondent was a health professional according to the laws of medicine and nursing and the laws of health care in the Republic of Croatia. Descriptive statistics were used for statistical analysis, and differences in the group of masters were determined by the χ2 test. All P values less than 0.05 were considered significant. IBM SPSS Statistics for Windows, version 25.0 was used for analysis and graphic display of the data. Results: 70% of the 400 planned surveys were completed. The study involved 279 respondents of both sexes and different ages. Results show healthcare professionals’ significant habit in intake of some dietary supplements, and appearance of COVID-19 affected a third of participants (11%) to start taking supplements, or to take them more frequently than before the pandemic (22%). During COVID-19 pandemic, healthcare professionals increased their intake of vitamin C, vitamin D, B vitamin complex, magnesium and zinc. Other supplements healthcare professionals increased their intake of are beta glucan, ginkgo biloba, collagen, and homeopathic preparations. Conclusion: Majority of healthcare professionals have a habit of consuming different dietary supplements, and appearance of COVID-19 pandemic caused statistically significant an increase in consumption.