Purpose: the objectives of this study were to analyse job satisfaction concerning years of work experience, age, qualifications and workplace amongst nurses and medical technicians in both hospitals, to analyse the satisfaction with advancement opportunities amongst the respondents in both hospitals, as well as to establish the correlation between job satisfaction and the superiors at workplace in both hospitals. Study design: a cross-sectional survey was conducted at the University Hospital Centre Zagreb and the University Hospital Merkur. Respondents and methods: the survey was conducted on 132 respondents, of whom 89 (67.4%) were employed at the University Hospital Centre Zagreb and 43 (32.6%) at the University Hospital Merkur. The respondents from both hospitals were employed at the Departments of Surgery. A questionnaire-scale was used as a research instrument to determine the general attitude and job satisfaction. Results: on the overall satisfaction scale, the respondents with the shortest work experience were significantly more satisfied, while those with longer work experience were significantly more dissatisfied. Overall satisfaction was significantly higher amongst the respondents from the University Hospital Centre Zagreb, the median stood at 117 (interquartile range from 107.5 to 125) (Mann Whitney U test, P = 0.007). The overall satisfaction score showed that 43 (33%) of the respondents were dissatisfied, 82 (62%) were either dissatisfied or satisfied, while only 7 (5%) respondents showed job satisfaction. Conclusion: the research study has shown that only 5% of nurses/medical technicians showed job satisfaction. Most respondents believed that they were not advancing as fast as in other professions and most of them were not satisfied with their career advancement opportunities.