Sage, laurel, myrtle, sea-buckthorn, carob, thyme and mastic trees are spices and herbs from the Mediterranean area with many potential applications due to the high content of biologically active compounds. Their plant extracts are used in the food and pharmaceutical industry, as well as in medicine. In order to obtain plant extracts with the highest possible content of bioactive compounds, it is crucial to choose the optimal extraction method. The aim of this study was to isolate, identify and quantify biologically active compounds of the studied plant extracts obtained by successive extraction with three different solvents (hexane, 80% acetone and 96% ethanol) in an ultrasonic bath. Identification and quantification of bioactive compounds were performed by high-performance liquid chromatography with UV-VIS/DAD detector and tandem mass spectrometry. Additionally, the antioxidant capacity of the plant extracts was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical. The mass fractions of carotenoids and chlorophylls in the extracts of analyzed plants were determined in the range of 25.12 mg 100 g dwˉ¹ in sea-buckthorn berries up to 97.60 mg 100 g dwˉ¹ in mastic tree leaves, while 96% ethanol was the most efficient solvent for the extraction of carotenoids and chlorophylls. On the other hand, the solvent with the highest efficiency for the extraction of polyphenolic compounds was 80% acetone and the mass fractions of polyphenolic compounds were ranging from 230.06 mg 100 g dwˉ¹ in carob pods up to 5356.29 mg 100 g dwˉ¹ in mastic tree leaves. In comparison with the content of carotenoids and chlorophylls, antioxidant activity was more in correlation with the content of total polyphenolic compounds. The highest antioxidant activity values were observed in the fractions with 80% acetone.