Flax cake is rich in proteins, but industrially it has been little used. Recently, it has become more and more interesting for research, due to the wealth of bioactive peptides with potential anti-oxidative effect, as well as the presence of other bioactive components. Considering the above, flax cake protein hydrolysates have the potential to be used in animal cell cultures as an addition to culture media. In doing so, they are used as a substitute for part of the serum, or simply as an additional ingredient that, due to its bioactive properties, can have a beneficial effect on cells in culture. In this work, the aim was to examine whether prepared protein hydrolysates from flax cake can have a protective effect on CHO DP-12 cells during induced oxidative stress. During the experiments, cells were inoculated in culture medium supplemented with different concentrations of flax protein hydrolysates in the range 0.1-5 g/L. Cells were counted using the Trypan Blue method, their viability was determined using the MTS method, and the obtained data were statistically processed. Through the experiment, it was concluded that flax hydrolysate obtained with the enzyme neutrase and added to the medium at a concentration of 0.5 g/L shows the greatest protective effect on CHO DP-12 cells exposed to oxidative stress, while flax hydrolysate obtained with the enzyme alcalase and added to the medium at a concentration of 0.1 g/L, shows a slightly inhibiting effect on cells exposed to oxidative stress. However, in order to confirm these conclusions, it is necessary to do more research on the same topic because there are very few works available for comparison in the literature.