Polyphenolic compounds have shown many potentially positive bioactivities like interactions with dietary fiber. Dietary fibers are resistant to digestion, and they can interact with polyphenols and carry them in their unchanged form to the lower parts of the digestive tract. There polyphenols can show their potentially positive activities. Interactions between polyphenols and dietary fibers can be interpreted through the adsorption process where polyphenols are adsorbates and dietary fibers are adsorbents. The aim of this work is to perform adsorption and desorption between polyphenol (naringin) and dietary fiber (zymosan A) in water, at pH 3.0, pH 7.0 and in electrolyte solutions for a simulated test in the stomach and small intestine. The influence of the type of solvent and the initial concentration of naringin and zymosan A on the adsorption process will also be investigated. The adsorption process was affected by the pH of the solution and the concentration of naringin and zymosan A. The adsorption capacity of naringin on zymosan A in water was from 142 to 718 \(mg g^{-1}\), at pH 3.0, from 16 to 518 \(mg g^{-1}\), at pH 7 ,0 from 14 to 780 \(mg g^{-1}\), in the electrolyte solution for the simulated test in the stomach from 140 to 929 \(mg g^{-1}\), and in the electrolyte solution for the simulated test in the small intestine from 38 to 647 \(mg g^{-1}\). The percentage of desorption for all tested solutions was from 0 to 95%, and recovery from 0 to 77%. The lowest percentage of desorption was in the solution for the simulated test in the stomach, and the highest in the solution for the simulated test in the small intestine. These interactions can be found in applications in the pharmaceutical industry, and to increase the bioavailability of naringin. Further research is needed to obtain more information about the same interactions.