The purpose of this research which was conducted in Samobor was to determine if there are differences and what are those differences in motor skills between boys and girls of preschool age. The research was conducted in a private kindergarten called "Osmijeh", in which twenty children took the motor skills test, of which there were ten boys and ten girls. The skills that were tested; balance, coordination, speed, explosive and repetitive strength, flexibility, and precision. Balance was measured by standing on one leg inside a circle. Coordination was measured by the backward obstacle course. Speed was measured by tapping the hand for 10 seconds. Strength was measured by a long jump and by lifting the torso on the floor (sit-ups in 30 seconds). Flexibility was measured by torso tilt in a narrow foothold. Precision was measured by shooting the ball at a given target. The test results showed significant differences in the measuring variable of explosive strength, flexibility, and speed. The boys achieved significantly better results in the long jump and hand tapping, while the girls achieved visibly better results in the torso tilt. And by measuring balance, coordination, and precision, no differences in motor abilities were found between boys and girls.