The concept of stress can be found in all professions, including in education. Stress is the name that explains the organism's reaction to stress, that is, environmental agencies that affect the functioning of the organism. The work of an educator is very specific and dynamic; therefore, it requires a high level of adaptability and emotional bearing in unpredictable situations, which can affect us not only as professionals, but also on a personal level. It is precisely in such situations that the educator's professionalism, composure, and rationality come to the fore. Leaving a child alone to nursery school is extremely stressful for the child, the parents, and the educator. Educators who put as much effort into their work are prone to stressful situations, and the long-term effect of stress can create physical, psychological, and social consequences. Negative behavior and stress of educators can lead to the appearance of burnout syndrome, which can become very serious if the cause of stress is not recognized in time, or if it is not eliminated in a timely manner. The aim of this diploma thesis is to investigate and gain insight into the sources of stress in kindergarten teachers. Furthermore, the goal is to investigate how certain sources of stress affect educators and what consequences they leave on them. The research method used was a focus group and an online survey via a Google form. The research sample consisted of 308 educators employed in kindergartens, who work in nurseries. The average experience of working in nurseries was five years. The most intensive stressors were those related to the health and safety of children of nursery age. The obtained research results will contribute to a better understanding of the work of educators and specific sources of stress for their working environment. They help in the further development of measures to identify sources of stress at work, their prevention, and their solutions.